Phosphorylation of Cdc25B3 Ser169 regulates 14-3-3 binding to Ser151 and Cdc25B activity
نویسندگان
چکیده
منابع مشابه
Phosphorylation of human keratin 18 serine 33 regulates binding to 14-3-3 proteins.
Members of the 14-3-3 protein family bind the human intermediate filament protein keratin 18 (K18) in vivo, in a cell-cycle- and phosphorylation-dependent manner. We identified K18 Ser33 as an interphase phosphorylation site, which increases its phosphorylation during mitosis in cultured cells and regenerating liver, and as an in vitro cdc2 kinase phosphorylation site. Comparison of wild-type v...
متن کاملAmino acids C-terminal to the 14-3-3 binding motif in CDC25B affect the efficiency of 14-3-3 binding.
The phospho-site adapter protein 14-3-3 binds to target proteins at amino acid sequences matching the consensus motif Arg-X-X-Ser/Thr-X-Pro, where the serine or threonine residue is phosphorylated and X is any amino acid. The dual-specificity phosphatase CDC25B, which is involved in cell cycle regulation, contains five 14-3-3 binding motifs, but 14-3-3 preferentially binds to the motif at Ser30...
متن کامل14-3-3 binding regulates catalytic activity of human Wee1 kinase.
The mitotic inducer Cdc2 is negatively regulated, in part, by phosphorylation on tyrosine 15. Human Wee1 is a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Human Wee1 is subject to multiple levels of regulation including reversible phosphorylation, proteolysis, and protein-protein interactions. Here we have investigated the contributions made by 14-3-3 binding to hum...
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The mammalian homologs of the C. elegans partitioning-defective (Par) proteins have been demonstrated to be necessary for establishment of cell polarity. In mammalian epithelia, the Par3/Par6/aPKC polarity complex is localized to the tight junction and regulates its formation and positioning with respect to basolateral and apical membrane domains. Here we demonstrate a previously undescribed ph...
متن کامل14-3-3 and regulation of Ataxin-1 function 14-3-3 Binding to Ataxin-1(ATXN1) Regulates its Dephosphorylation at S776 and Transport to the Nucleus
Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455. Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota 55455. Departments of Molecular and Human Genetics, Pediatrics, and Howard Hughes Medi...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2011
ISSN: 1538-4101,1551-4005
DOI: 10.4161/cc.10.12.15819